Volume 10 ; Issue 2 ; in Month : July-Dec (2026) Article No : 210
Kumar S, Kumar P, Fazal B

Abstract

Background: Ibuprofen is a widely used non-steroidal anti-inflammatory drug for the management of pain, inflammation and fever. Simple analytical procedures remain useful for routine pharmaceutical laboratories, particularly where chromatographic instrumentation is not readily available. The present study developed and evaluated a direct UV–visible spectrophotometric procedure for quantitative estimation of ibuprofen. Methods: Ibuprofen was dissolved in methanol and analyzed using a double-beam UV–visible spectrophotometer with 1-cm matched quartz cells. The absorption spectrum was recorded from 200–400 nm and the analytical wavelength was selected from the observed maximum. Linearity was assessed at 2–12 µg/mL. Accuracy was investigated at 80%, 100% and 120% levels; repeatability, intraday and interday precision, robustness to wavelength variation, and sensitivity were also evaluated. Results: Ibuprofen showed a maximum absorption at 222 nm. The project-reported calibration equation was y = 0.060x − 0.001 with R² = 0.9994 over 2–12 µg/mL. Recovery ranged from 99.50% to 100.30%, with a mean recovery of 99.77%. Reported %RSD values for repeatability, intraday and interday precision were 0.61%, 0.78% and 1.04%, respectively. Absorbance values at 220, 222 and 224 nm were 0.592, 0.598 and 0.595, respectively. Reported LOD and LOQ were 0.16 and 0.49 µg/mL. Conclusion: The evaluated procedure showed strong linear response, satisfactory recovery, low variability and tolerance to small wavelength changes. It therefore represents a rapid and economical procedure for quantitative analysis of ibuprofen standard solutions. Before claiming routine assay of finished dosage forms, formulation-specific selectivity and assay data should be generated.

Full Text Attachment

Views : 2      Downloads : 1

RSS